KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0487 | - |
3-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.0559 | - |
2-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.0667 | - |
2-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.0813 | - |
cis,cis-muconate | - |
Rhodococcus erythropolis | |
1.24 | - |
3-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | 250000 | nondenaturing electrophoresis, gel filtration | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
1CP | - |
Rhodococcus erythropolis 1CP | - |
1CP | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-chlorophenol-grown cell | - |
Rhodococcus erythropolis | - |
culture condition:benzoate-grown cell | - |
Rhodococcus erythropolis | - |
Storage Stability | Organism |
---|---|
-20°C, enzyme concentration 0.0035 mg/ml, 50 mM MOPS buffer, pH 6.5, 26% loss of activity after 2 months | Rhodococcus erythropolis |
20°C, enzyme concentration 0.0035 mg/ml, 50 mM MOPS buffer, pH 6.5, 69% loss of activity after 2 months | Rhodococcus erythropolis |
4°C, enzyme concentration 0.0035 mg/ml, 50 mM MOPS buffer, pH 6.5, no loss of activity after 2 months | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
2-Chloro-cis,cis-muconate | - |
Rhodococcus erythropolis 1CP | ? | - |
? | |
2-Methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
2-Methyl-cis,cis-muconate | - |
Rhodococcus erythropolis 1CP | ? | - |
? | |
3-Chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | 5-Chloromuconolactone | dehalogenation is not catalyzed | ? | |
3-Chloro-cis,cis-muconate | - |
Rhodococcus erythropolis 1CP | 5-Chloromuconolactone | dehalogenation is not catalyzed | ? | |
3-Methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | beta-Methylmuconolactone | - |
? | |
3-Methyl-cis,cis-muconate | - |
Rhodococcus erythropolis 1CP | beta-Methylmuconolactone | - |
? | |
cis,cis-Muconate | - |
Rhodococcus erythropolis | (+)-Muconolactone | - |
? | |
cis,cis-Muconate | - |
Rhodococcus erythropolis 1CP | (+)-Muconolactone | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000-42000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 17% loss of activity | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
3-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
3.2 | - |
3-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
3.6 | - |
2-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
8.8 | - |
3-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
24.7 | - |
cis,cis-muconate | - |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus erythropolis |